News

December 2018

WITec Establishes New Partnership with Elliot Scientific for UK and Ireland

WITec is pleased to announce a new partnership for the sales and support of its products in the UK and Ireland. The cooperation with Elliot Scientific Limited, an established supplier of opto-mechanical components, will enhance the local visibility of WITec’s line of Raman imaging and scanning probe microscopes and ensure closer contact with its customer base. Managing Director of Elliot Scientific Dr. Adrian Knowles, who has extensive Raman spectroscopy experience along with an existing working relationship with WITec, will take the lead in promoting sales activities and providing support for new and installed user systems in the UK and Ireland. The combined talents of both companies will serve to further WITec’s position as the leading manufacturer of confocal Raman and correlative microscopy systems.

For more information, please visit the website>>>

显示更多...

November 2018

WITec Releases 5.1 Software Update

WITec’s powerful software suite has been updated with release 5.1. For WITec Control users this new version features Focus Stacking, which can stitch together sample area images acquired at different focal depths into larger images of consistent sharpness, and Smart Brightness, which automatically adjusts the camera settings and microscope illumination to match a user-defined brightness in the video image. The new version of WITec Project contains new Lighting Effects, an Animation Editor and a Stack Bitmap Export function.

For more information, please visit the product webpage>>>

A measurement of a housefly's eye. The upper row shows the images from several focal depths and the larger composite is a result of Focus Stacking.

显示更多...

October 2018

Konferenzrückblick: 15. Confocal Raman Imaging Symposium in Ulm

Wie ein großes Familientreffen mit bekannten und neuen Gesichtern war das Confocal Raman Imaging Symposium, das zum 15. Mal in Ulm stattfand. Der Ulmer Mikroskophersteller WITec hatte Forscher, Ingenieure und Wissenschaftler vom 24. bis 26. September 2018 eingeladen, sich über die neuesten Entwicklungen der konfokalen Raman Mikroskopie auszutauschen.

Mit über 100 Teilnehmern aus zahlreichen Ländern war die internationale Konferenz auch in diesem Jahr sehr gut besucht. Experten und Einsteiger nehmen seit Jahren die Möglichkeit war, ihr Wissen über moderne Raman Mikroskopie aufzufrischen und zu erweitern. Dafür gab es genügend Gelegenheit bei Vorträgen und Posterbeiträgen, aber auch im direkten Gespräch mit anderen Konferenzteilnehmern. Namhafte Sprecher aus Wissenschaft und Industrie informierten das Publikum anschaulich über Forschungsergebnisse in ihrem Fachgebiet. Zudem konnten die Teilnehmer ihre Arbeiten auf Postern präsentieren und miteinander diskutieren. Ergänzt wurde das Programm durch Vorführungen der neuesten konfokalen Raman Mikroskope. Auch der Humor kam beim Abendvortrag des Wissenschafts-Komikers und Physikers Vince Ebert nicht zu kurz, der es schaffte, das internationale Publikum zum Lachen zu bringen.

Zum Auftakt des Symposiums hielt Sebastian Schlücker (Universität Duisburg-Essen, Deutschland) einen Vortrag über die theoretischen Grundlagen der Raman Spektroskopie und gab Einblicke in spezielle Raman Techniken zur Erhöhung der Auflösung und Verstärkung des Raman Signals. Abschließend ließ Schlücker die Zuhörer ihr Wissen in einem interaktiven Quiz testen.
Olaf Hollricher (WITec GmbH, Ulm, Deutschland) informierte über die technischen Möglichkeiten der Raman Mikroskope und erklärte, wie durch die passenden Geräte-Konfigurationen die Ergebnisse verbessert werden können.
Den Abschluss der wissenschaftlichen Vorträge am ersten Tag machte Katarzyna Marzec (Jagiellonian Universität, Krakau, Polen) mit einer Präsentation ihrer Arbeit über korrelative Raman Mikroskopie in der biomedizinischen Forschung. Marzec und Kollegen verwenden die konfokale Raman Mikroskopie in Kombination mit Rasterkraft-, Nahfeld- oder Fluoreszenz-Mikroskopie. Sie analysieren biologische Gewebe und Zellen, um Erkrankungen wie Arteriosklerose, Krebs und Malaria besser zu verstehen und behandeln zu können.

Der zweite Symposiumtag startete mit der Vortragsreihe Nanotechnologie und neue Materialien. In den Vorträgen von Dirk Guldi (Universität Erlangen, Deutschland) und Bo Peng (Universität für Elektronische Wissenschaft und Technologie, Chengdu, China) wurden Forschungsarbeiten zur Verbesserung der Materialeigenschaften von Graphen, Wolframdisulfid und Molybdändisulfid behandelt. Diese leitenden Nanomaterialien sollen zukünftig in elektronischen Geräten Anwendung finden.

Zu Beginn der geowissenschaftlichen Vortragsreihe stellte Guillaume Wille (BRGM Orleans, Frankreich) seine Untersuchungen an polymorphen Strukturen und Asbest mittels korrelativer Raman Imaging und Scanning Electron (RISE) Mikroskopie vor. Dabei verbindet er die elektronenmikroskopischen Erkenntnisse über Proben-Morphologie und -Nanostruktur mit den Informationen zur chemischen Mineralzusammensetzung, die er durch die Raman Mikroskopie erhält. Durch seine Untersuchungen an Asbest können Gesundheitsrisiken besser erkannt und verhindert werden.
Dominic Papineau (London Center for Nanotechnology, UK) zeigte seine umfassenden Forschungsergebnisse geologischer Proben. Sein Interesse gilt dem Nachweis organischer Verbindungen im Gestein, um das urzeitliche Auftreten mikrobiellen Lebens genauer zu datieren. Mit der Raman Mikroskopie konnten Papineau und Kollegen kürzlich eines der ältesten Mikrofossilien identifizieren.
Ein breites Spektrum unterschiedlicher Proben analysiert Keith Gordon (Universität Otago, Dunedin, Neuseeland), der Proben wie Fasern archäologischer Maori-Funde und hypomineralisierte Zähnen chemisch analysiert.

In der Vortragsreihe Materialwissenschaften berichtete Martin Maiwald (Ferdinand-Braun-Institut, Berlin, Deutschland) über SERDS, einer Methode mittels zweier leicht unterschiedlicher Anregungswellenlängen störende Hintergrund- und Fluoreszenzsignale aus dem Raman-Spektrum zu entfernen und dadurch bessere Ergebnisse zu erzielen.
Jagjit Nanda (Oak Ridge National Laboratory, USA) forscht seit vielen Jahren über Batterien und konnte umfangreiche Raman Analysen zum Ladezustand von Lithium-Ionen-Batterien präsentieren.
Christian Timma (thyssenkrupp Steel Europe AG, Duisburg, Deutschland) berichtete anschaulich über die Etablierung der konfokalen Raman Mikroskopie zur Qualitätskontrolle bei der Stahl-Herstellung in seinem Unternehmen.

In der Vortragsreihe Life Science erläuterte Isaac Pence (Imperial College London, UK) seine Forschungsergebnisse an künstlich hergestelltem Knorpelgewebe. Pence und Kollegen möchten ein genaues Bild der chemischen und molekularen Bestandteile des Gewebes erhalten, um den Eigenschaften natürlichen Gewebes möglichst nahe zu kommen. Auch Unterschiede in der Lipid-Zusammensetzung der Myelin-Schicht zwischen normalem Nervengewebe und Gewebe von Mäusen mit Multipler Sklerose analysiert Pence mit der Raman Mikroskopie. Außerdem stellte er die SPARTA-Methode vor, mit der kleine Nanopartikel in Lösung mittels Raman Spektroskopie ohne Anfärben analysiert werden können.
Dieter Baurecht (Universität Wien, Österreich) erzählte über das breite Spektrum an Fragestellungen, die an sein Labor aus verschiedenen Fachbereichen herangetragen werden, und die er mittels konfokaler Raman Mikroskopie und AFM zu lösen versucht.

Im Anschluss folgten die Kurzpräsentationen, die eine Jury aus den eingereichten Zusammenfassungen der Forschungsarbeiten der Symposium-Teilnehmer ausgewählt hatte. Hesham K. Yosef (Ruhr Universität Bochum, Deutschland) hielt einen Vortrag über pharmakokinetische Untersuchungen an Lungen- und Brustkrebszellen. Er analysiert mit korrelativer Raman-Fluoreszenz Mikroskopie die Aufnahme und Umsetzung des Krebsmedikaments Neratinib in Zellkulturen und konnte zeigen, dass Rezeptoren, die für das Zellwachstum wichtig sind, Neratinib-induziert abgebaut werden. Eva Brauchle (Universität Tübingen, Deutschland) stellte ihre Arbeit über Parkinsonerkrankungen vor. Mittels Raman Imaging untersucht sie veränderte Proteinstrukturen und Aggregatbildung im neuronalen Gewebe von Ratten. Zukünftig könnte ihre Forschung dazu beitragen, frühzeitig Informationen über den Krankheitsverlauf zu erhalten. Vinayam B. Parambath (HIU, Ulm, Deutschland) untersucht Auf- und Entlademechanismen von Metall-Schwefel-Batterien, um diese in Zukunft effizienter zu machen.

Am Abend fand das traditionelle Konferenz-Dinner im Ratskeller in Ulm statt. Dort wurde auch der Poster Award verliehen. Die Jury entschied sich dieses Jahr für Dieter Fischers (Leibniz-Institut für Polymerforschung, Dresden, Deutschland) Poster über Mikroplastik-Partikel-Analysen.

Als Resümee des Symposiums gaben die Teilnehmer an, dass sowohl die Inhalte als auch die Durchführung des Symposiums überzeugt haben. Viele haben bereits für nächstes Jahr ihre Teilnahme angekündigt.

The attendees of the 15th Confocal Raman Imaging Symposium.

显示更多...

June 2018

International Conference on Raman Imaging in its 15th Year

For the 15th time, WITec’s Confocal Raman Imaging Symposium will serve as the foremost platform for the Raman microscopy community to share recent developments and discuss the finer points of the technique. The annual international conference for chemical characterization and imaging will be held from September 24th to the 26th, 2018 in Ulm, Germany.

Raman Symposium Attendees 2017

The attendees of the last conference 2017.

The Symposium is a well-established forum for scientists from academia and industry of various levels of experience and areas of application. The conference schedule includes talks, poster sessions, instrument demonstrations and social events to foster the transfer of knowledge and experience between the conference attendees.

Lecture sessions in the fields of nanotechnology and low-dimensional materials, geo sciences, materials sciences, life sciences and pharmaceutics will provide a vivid overview of Raman microscopy applications. The latest developments in Raman instrumentation and technology will also be covered extensively.

2017 Vince Ebert Speaker 1 Michael Zargarinejad

Vince Ebert, physicist and comedian, will give the evening lecture at the second day of the symposium.

A special item on the conference schedule is the evening lecture of Vince Ebert, a German physicist and science comedian, who will illuminate the humorous side of science.

For more information on the conference program, speakers and registration, please visit: www.raman-symposium.com

The conference venue is located next to the Muenster in the heart of Ulm, Germany.

显示更多...

May 2018

2018 WITec Paper Award for Outstanding Scientific Publications

Every year WITec, the Raman imaging company, recognizes three peer-reviewed publications that stand out in terms of originality and significance and feature data acquired with a WITec microscope. This year, scientists from universities and institutes in Bochum, Bayreuth and Minneapolis received the awards. Some 80 publications were submitted, all documenting the versatility of confocal Raman imaging for the investigation of materials.

 

The Gold Paper Award

The Gold Paper Award goes to Hesham K. Yosef. With his colleagues from the Department of Biophysics, headed by Klaus Gerwert, and coworkers from other institutes at Ruhr-University Bochum (Germany) he developed a new, non-invasive approach based on Raman imaging for the detection of urothelial carcinoma cells in urine. Urothelial carcinomas constitute the majority of bladder tumors. Noninvasive, painless methods are urgently wanted for the diagnosis of this cancer and the monitoring of the treatment outcome. The scientists first identified differences in the Raman spectra of healthy and high-grade cancerous cells in urine. The most prominent differences in Raman bands indicated a decrease in the level of glycogen and an increase in the levels of fatty acids in the tumor cells, illustrating distinct metabolic changes. The scientists then developed and trained a classifier that could differentiate healthy from cancerous urothelial cells by their Raman spectra. A Raman band at 482 relative wavenumbers, indicating a massive loss of glycogen in tumor cells, emerged as a highly reliable marker. A first validation test on the urine of ten diagnosed and ten healthy patients was performed and the classifier diagnosed all samples automatically with 100% accuracy. First author of the publication, Hesham K. Yosef, says that he is convinced that, “The results will pave the way for spectral, noninvasive cytology of urine using Raman microspectroscopy.”

 

The Silver Paper Award

Mimicking biological structures with interesting functional features is a thriving field of research. Marvin Gernhardt, Holger Schmalz, Seema Agarwal and coworkers at the Chair of Macromolecular Chemistry II, University of Bayreuth, and the Bavarian Polymer Institute (Germany) were inspired by biological fibers and developed new fibers with distinct structures. They produced the fibers by side-by-side electrospinning of a thermo-responsive polymer and methacrylate-based copolymer. By changing the spinning solvents they fabricated side-by-side and coaxial bead-on-string fibers and analyzed these in detail with correlative Raman imaging, atomic force microscopy and scanning electron microscopy. The scientists concluded: “The work opens the way for the construction of heterostructured fiber morphologies based on different polymer combinations, offering high potential for applications as actuators, smart textiles, water management and catalysis.”

 

The Bronze Paper Award

Guanglin Yu receives the Bronze Paper Award. He and his colleagues from the Departments of Mechanical Engineering and Biomedical Engineering at the University of Minnesota in Minneapolis (USA) analyzed, when and how ice crystals develop in cells during freezing. Cryopreservation is a common method used to store cells, however not all cells survive the procedure. It has been hypothesized that a high cooling rate leads to the death of cells because of intercellular ice formation. How that happens is still a matter of debate. The researchers used confocal Raman imaging to monitor the freezing response of lymphoblasts under various conditions during the cryopreservation process. Ice crystals, cell mass and protective substances could be easily imaged and identified by their Raman spectra. As a parameter of cell death, they used a Raman signal indicating the release of cytochromec from mitochondria. The imaging study showed that only the formation of large intracellular ice crystals (> 2 μm) is deadly for the cells and that a mixture of glycerol and trehalose prevented cell death most efficiently at a cooling rate of 10°C per minute. “Raman spectromicroscopy provides a powerful tool for observing IIF [intracellular ice formation] and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols,” concluded the authors in their paper.

 

2018 WITec Paper Award Winners

GOLD: Hesham K. Yosef, Sascha D. Krauß, Tatjana Lechtonen, Hendrik Jütte, Andrea Tannapfel, Heiko U. Käfferlein Thomas Brüning, Florian Roghmann, Joachim Noldus, Axel Mosig, Samir F. El-Mashtoly and Klaus Gerwert: Noninvasive diagnosis of high-grade urothelial carcinoma in urine by Raman spectral imaging. Analytical Chemistry 89, 6893 (2017), DOI 10.1021/acs.analchem.7b01403.

SILVER: Marvin Gernhardt, Ling Peng, Matthias Burgard, Shaohua Jiang, Beate Förster, Holger Schmalz and Seema Agarwal: Tailoring the morphology of responsive bioinspired bicomponent fibers. Macromolecular Materials and Engineering 303, 1700248 (2017), DOI. 10.1002/mame.201700248.

BRONZE: Guanglin Yu, Yan Rou Yap, Kathryn Pollock and Allison Hubel: Characterization intracellular ice formation of lymphoblasts using low-temperature Raman spectroscopy. Biophysical Journal 112, 2653 (2017), DOI 10.1016/j.bpj.2017.05.009.

 

The WITec Paper Award is an annual contest sponsored by WITec and awarded to author(s) to recognize his or her outstanding peer-reviewed publication featuring results acquired with a WITec instrument. Scientists from all fields of application in both academia and industry are invited to submit their publications to papers@witec.de. The deadline for submissions for the next Paper Award is January 31st, 2019.

First author Hesham Yosef (left) and senior author Klaus Gerwert receive the winner’s certificate from WITec’s marketing assistant Karin Hollricher. Copyright: RUB, Marquard

显示更多...

May 2018

Confocal Raman Microscopy 2nd Edition – At bookstores now

Confocal Raman Microscopy, edited by members of the WITec team and part of the Springer Series in Surface Sciences, has been thoroughly revised and expanded for its 2nd Edition. The updated book is available now and features the latest developments in Raman techniques and instrumentation.

As with the 1st Edition, which has already established itself as the preeminent resource for the technique in labs and libraries around the world, the new edition provides a comprehensive overview of the fundamentals, practical considerations and real-world applications of Raman microscopy. Additionally, it has nearly doubled in number of chapters and now includes sub-sections on theory and technology, novel materials, geosciences, life and pharmaceutical sciences, materials science and a historical overview of the Raman effect. It also explores the rapidly evolving field of correlative microscopy by detailing how 3D Raman imaging can be integrated with other investigative methods to achieve a far greater understanding of a sample’s properties.

The 2nd Edition of Confocal Raman Microscopy, edited by WITec scientists Dr. Olaf Hollricher and Dr. Jan Toporski, can be purchased in print or e-book formats directly from Springer or through online shops. Order your copy today and have the current state of the art in Confocal Raman Microscopy at your fingertips.

Confocal Raman Microscopy
Editors: Jan Toporski, Thomas Dieing, Olaf Hollricher
ISBN: 978-3-319-75380-5

显示更多...

March 2018

alpha300 Ri - New inverted confocal Raman microscope

WITec’s proven 3D Raman imaging functionality is now available in an inverted microscope.

WITec has launched the alpha300 Ri inverted Raman microscope. It combines the advantages of data acquisition from below with the established merits of 3D confocal Raman imaging, a powerful and versatile technique that can chemically characterize samples nondestructively and without labeling or other specialized preparation. The speed, sensitivity and resolution of the WITec alpha300 series are now available from a new angle. Research applications in the fields of life sciences, biomedicine and pharmaceutics will benefit in particular from the new setup.

The inverted beam geometry of the alpha300 Ri delivers many advantages in sample access and handling. Specimens in aqueous environments such as cell cultures can be examined more effectively. Standardized liquid sample holder formats can be quickly and easily mounted and measured. This accelerates the experimental workflow and helps ensure consistency. Investigations in materials science will be aided by the very large working area that can accommodate bulky samples and the set focal plane. The motorized sample stage also facilitates the mounting of environmental enclosures and other accessories.

Many modular components and upgrade possibilities developed for the WITec alpha300 series are compatible with the Ri version. Other microscopy techniques associated with inverted microscopes, such as fluorescence, differential interference contrast (DIC) and phase-contrast can also be easily integrated.

According to Olaf Hollricher, Director of Research and Development at WITec, “Researchers in life sciences are accustomed to working with inverted microscopes and their advantages for in vivo measurements, among others, are well-established. Developing a variant of our alpha300 confocal Raman imaging series with an inverted beam is a logical step in the evolution of our product line. Now the benefits of nondestructive, label-free molecular characterization are available from below the sample plane, backed up by all the modularity and upgradeability inherent in our systems.”

The WITec alpha300 Ri will be showcased at Analytica 2018 in Munich at the WITec booth A2/402. For more information, please visit the product webpage >>>.

显示更多...

March 2018

WITec Releases TrueMatch - Integrated Raman Spectral Database Software

WITec, manufacturer of 3D Raman imaging systems, is now offering TrueMatch, a powerful software component for accessing and creating Raman spectral databases. It can search existing libraries of Raman spectra to identify sample components and also allows users to create their own catalog of acquired spectra to assist in their research.

TrueMatch is available as a fully integrated option with the WITec Project FIVE software environment and databases can be brought directly into use in post-processing. Multi-spectral searches enable the user to identify several spectra simultaneously, and return hit rates that show the probability of a match for each individual spectrum. This greatly accelerates the process of characterization and is a perfect complement to WITec’s standard data analysis features. Demix search uses databases to describe acquired spectra in terms of pure substances, which can then be physically located in an image. Seeing the distribution of pure chemical components matched to sample features gives the researcher a precise visualization. Generated reports can be stored for further use in subsequent measurements, amplifying the effectiveness of the system as a whole and turning meaningful results into a valuable resource.

In addition to the proprietary WITec Raman database capability included with TrueMatch, compatible versions of ST Japan’s wide range of databases are available. Extensive libraries of Raman spectra can be referenced while new ones are created, all from within WITec’s fully-integrated software and hardware environment.

“TrueMatch makes it easier for the user to interpret their Raman images. It also greatly simplifies the process of searching through Raman spectral databases and creating new ones based on user-generated data compared to anything else out there. It’s the perfect complement to the standard analysis methods WITec implements in our Suite FIVE software environment,” says Olaf Hollricher, Managing Director and Director of Development at WITec, in summarizing the advantages of the new software.

Visit the TrueMatch product page for detailed information on the new software.

 

TrueMatch is a powerful and innovative software component for accessing and developing Raman spectral databases

显示更多...