Knowledge Base

What influences the throughput of a confocal Raman microscope?

The throughput of a confocal Raman microscope has a direct impact on the amount of time needed for confocal Raman imaging and the sensitivity and capability of the system. It is influenced by various microscope components, such as the objective, coupling elements and filters, the pinhole, the spectrometer, and the CCD camera. To optimize the throughput, every part of the system must be optimized for the highest transmission and efficiency.

high-throughput system low-throughput system
high quality objective low quality objective
beam guidance using a single fiber beam guidance using mirrors (e.g. 3 mirrors)
lens-based spectrometer mirror-based spectrometer
back-illuminated CCD camera front-illuminated CCD camera

 

WITec High Low Throughput systems

High-throughput system optimized for 532 nm excitation wavelength vs. low-throughput system. Throughput limitations due to the pinhole are neglected for both cases.